Fluorescence-Based Methods for the Determination of Selected β-Lactam Antibiotics (Amoxicillin, Cefixime, and Cephalexin): A Review

Authors

• Kardos Musheer Khorsheed, Fuel and Energy Techniques Engineering, College of Oil and Gas Techniques Engineering, Northern Technical University, Kirkuk 36001, IraqFollow
• Tara Fuad Tahir, Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya 44023, Kurdistan Region., Iraq
• Ahmad F. Hama Sdiq, Department of Medical Laboratory Science, College of Health Sciences, Lebanese French University, 44001 Erbil, Kurdistan Region, Iraq

How to Cite This Article

Khorsheed, Kardos Musheer; Tahir, Tara Fuad; and Hama Sdiq, Ahmad F. (2026) "Fluorescence-Based Methods for the Determination of Selected β-Lactam Antibiotics (Amoxicillin, Cefixime, and Cephalexin): A Review," Polytechnic Journal: Vol. 16: Iss. 2, Article 1.
DOI: https://doi.org/10.59341/2707-7799.1879

Document Type

Review

Abstract

β-Lactam antibiotics constitute one of the most extensively prescribed classes of antimicrobial agents in human and veterinary medicine. However, their widespread use has raised growing concerns regarding antibiotic resistance, drug safety, and the presence of residual antibiotics in pharmaceutical products, biological fluids, foodstuffs, and environmental samples. As a result, the development of sensitive, selective, and rapid analytical methods for the determination of β-lactam antibiotics has become critically important. Among the available analytical techniques, fluorescence-based methods have gained increasing attention due to their high sensitivity, operational simplicity, low cost, and suitability for trace-level detection. This review presents a comprehensive overview of fluorescence-based approaches for the determination of representative β-lactam antibiotics, with particular emphasis on amoxicillin, cefixime, and cephalexin. Various fluorometric strategies including direct fluorescence, derivatization-based assays, fluorescence quenching and enhancement mechanisms, ratiometric sensing, and nanomaterial-assisted platforms such as quantum dots, carbon dots, metal–organic frameworks, and molecularly imprinted polymers are critically discussed. The analytical performance of reported methods is compared in terms of linear dynamic range, limits of detection, selectivity, and applicability to real samples. Overall, this review highlights the significant potential of fluorescence-based techniques for reliable β-lactam antibiotic analysis in pharmaceutical quality control, food safety monitoring, and environmental assessment.

Receive Date

16 May 2026

Revise Date

25 June 2026

Accept Date

12 July 2026

Publication Date

9-1-2026

References

[1] Ofori-Asenso R, Agyeman AA. Irrational use of medicines―a summary of key concepts. JP 2016;4(4):35. https:// doi.org/10.3390/pharmacy4040035.

[2] Ventola CL. The antibiotic resistance crisis: part 1: causes and threats (in eng). JP Ther 2015;40(4):277—83.

[3] Prescott JF. Beta-lactam antibiotics: cephalosporins. JAtivm 2013:153—73. https://doi.org/10.1002/9781118675014.ch9.

[4] Vilvanathan S. Penicillins, cephalosporins, and other β- Lactam antibiotics. Introduction to basics of pharmacology and toxicology: volume 2: Essentials of systemic pharmacology: from principles to practice. Springer; 2021. p. 821—34. https://doi.org/10.1007/978-981-33-6009-9.

[5] Luo Y, Sun Y, Wei X, He Y, Wang H, Cui Z, et al. Detection methods for antibiotics in wastewater. Rev 2024;47(9): 1433—51. https://doi.org/10.1007/s00449-024-03033-0.

[6] Getahun H. Antimicrobial resistance. Global health essentials. Springer; 2023. p. 143—7. https://doi.org/10.1007/978-3- 031-33851-9_22.

[7] Ghimpețeanu OM, Pogurschi EN, Popa DC, Dragomir N, Dragotoiu T, Mihai OD, et al. Antibiotic use in livestock and residues in food―a public health threat: a review. Foods 2022;11(10):1430. https://doi.org/10.3390/foods11101430.

[8] Oliveira N, Gonçalves B, Lee S, Oliveira C, Corassin C. Use of antibiotics in animal production and its impact on human health. J Food Chem Nanotechnol 2020;6(1):40—7. https:// doi.org/10.17756/jfcn.2020-082.

[9] Talebi Bezmin Abadi A, Rizvanov AA, Haertle T, Blatt NL. World Health Organization report: current crisis of antibiotic resistance. JB 2019;9(4):778—88. https://doi.org/10. 1007/s12668-019-00658-4.

[10] Gootz TDJCRiI. The global problem of antibiotic resistance. 2010. https://doi.org/10.1615/CritRevImmunol.v30.i1.60. 30(1).

[11] Geddes AM, Gould IM. Ampicillin, amoxicillin and other ampicillin-like penicillins. JKtuoateLHA 2010:65.

[12] Brockow K, Przybilla B, Aberer W, Bircher A, Brehler R, Dickel H, et al. Guideline for the diagnosis of drug hypersensitivity reactions. 2016. https://doi.org/10.1007/ s40629-015-0052-6.

[13] A Adefegha. Antibiotics and drug pharmacology. JASPS 2019;3:43—9. https://doi.org/10.31080/ASPS.2019.03.0424.

[14] Meng X, Earnshaw CJ, Tailor A, Jenkins RE, Waddington JC, Whitaker P, et al. Amoxicillin and clavulanate form chemically and immunologically distinct multiple haptenic structures in patients. 2016. p. 1762—72. https://doi.org/10. 1021/acs.chemrestox.6b00253. 29(10).

[15] Kaur SP, Rao R, Nanda S. Amoxicillin: a broad spectrum antibiotic. JIJPPS 2011;3(3):30—7.

[16] Davies J, Davies D. Origins and evolution of antibiotic resistance. JM Reviews mb 2010;74(3):417—33. https://doi. org/10.1128/mmbr.00016-10.

[17] Prasetyawan F, Saristiana Y, Mildawati R, Rhomah EH, Fadel MN, Besan Ejj. Pharmacokinetics and druglikeness of amoxicillin drug using swissADME. MIJoM 2025;1(2):67—75. https://doi.org/10.64123/mijm.v1.i2.3.

[18] Furuta T, Sugimoto M, Yamade M, Uotani T, Sahara S, Ichikawa H, et al. Effect of dosing schemes of amoxicillin on eradication rates of Helicobacter pylori with amoxicillin- based triple therapy. 2014. p. 258—66. https://doi.org/10. 1002/jcph.195. 54(3).

[19] Mancano MA, Gallagher JC. Frequently prescribed medications: drugs you need to know. Jones & Bartlett Publishers; 2013.

[20] Gillies M, Ranakusuma A, Hoffmann T, Thorning S, McGuire T, Glasziou P, et al. Common harms from amoxicillin: a systematic review and meta-analysis of randomized placebo-controlled trials for any indication. 2015. p. E21—31. https://doi.org/10.1503/cmaj.140848. 187(1).

[21] Shenoy ES, Macy E, Rowe T, Blumenthal KG. Evaluation and management of penicillin allergy: a review. JJ 2019; 321(2):188—99. https://doi.org/10.1001/jama.2018.19283.

[22] Veeraraghavan B, Bakthavatchalam YD, Sahni RD. Orally administered amoxicillin/clavulanate: current role in outpatient therapy. JID Ther 2021;10(1):15—25. https://doi. org/10.1007/s40121-020-00374-7.

[23] Bonomo RA. Multiple antibiotic-resistant bacteria in long- term-care facilities: an emerging problem in the practice of infectious diseases. JCID 2000;31(6):1414—22. https://doi.org/ 10.1086/317489.

[24] Arumugham VB, Gujarathi R, Cascella M. Third-generation cephalosporins. StatPearls Publishing; 2023. StatPearls [Internet].

[25] Guay D. Cefixime. Kucers' the use of antibiotics. CRC Press; 2017. p. 539—47. https://doi.org/10.1201/9781498747950.

[26] Sergey NA, Svistushkin V, Alexander IS, Zaitseva O, Zakharova I, Zyryanov S, et al. Cefixime (advanced oral third-generation cephalosporin) and its place in clinical practice. 2025. p. 42—50. https://doi.org/10.36488/cmac.2025. 1.42-50. 27(1).

[27] Choudhari S, Pawar D, Bhure A, Ade AJ. CefixView: understanding trends in respiratory infections. IJoB Pharm C 2024; 13(5):685. https://doi.org/10.18203/2319-2003.ijbcp20242429.

[28] Hamilton-Miller J. Overview of cefixime use in community- acquired infections. JCM Inf 2000;6:79—81. https://doi.org/ 10.1111/j.1469-0691.2000.tb02051.x.

[29] Shafi MK, Shah AA, Khan MA, Faisal S, Iqbal S, Shafi MK, et al. The assessment and efficiency of cefixime in upper respiratory tract infections: insights and perspectives. 2024. https://doi.org/10.7759/cureus.64539. 16(7).

[30] Sudharsan H, Ta K, Ali M. A review on the use of third- generation cephalosporins on gram-positive and gram- negative bacteria based on its spectrum of activity. JRTPSR 2024;6(1):1—7. https://doi.org/10.46610/RTPSR.2024.v06i01. 001.

[31] Shalaan AH, Muhammed AJ, Mohammed RM, Ahmed SJ. The effects of cephalosporin antibiotics generations on samples taken from different cases (oral cavity and surgical wounds).

[32] Nayeem A, Suresh AS, Vellapandian C, Singh S, Elossaily GM, Prajapati BG. Comprehensive insights into cephalosporins: spectrum, generations, and clinical applications. JCDT 2024. 0115748855339119241204071146. https://doi.org/10.2174/

[33] Lyu Y. The Development of cephalosporin: different generation. JWSRJ 2021;7(1):365—72. https://doi.org/10.6911/ WSRJ.202101_7(1).0046.

[34] Moran GJ, Krishnadasan A, Mower WR, Abrahamian FM, LoVecchio F, Steele MT, et al. Effect of cephalexin plus trimethoprim-sulfamethoxazole vs cephalexin alone on clinical cure of uncomplicated cellulitis: a randomized clinical trial. 2017. p. 2088—96. https://doi.org/10.1001/jama. 2017.5653. 317(20).

[35] Bisno AL. Are cephalosporins superior to penicillin for treatment of acute streptococcal pharyngitis? JCID 2004; 38(11):1535—7. https://doi.org/10.1086/392520.

[36] Jacqz-Aigrain E, Choonara I. The administration of medicines to children. Paediatric clinical pharmacology. CRC Press; 2021. p. 217—73.

[37] Giordano PA, Elston D, Akinlade BK, Weber K, Notario GF, Busman TA, et al. Cefdinir vs. cephalexin for mild to moderate uncomplicated skin and skin structure infections in adolescents and adults. 2006. p. 2419—28. https://doi.org/ 10.1185/030079906X148355. 22(12).

[38] Bose A, Thomas I, Abraham E. Fluorescence spectroscopy and its applications: A Review. JIJAPR 2018;8(1):1—8. https:// doi.org/10.7439/ijapa.v8i1.4578.

[39] Wolfbeis OS. Fluorescence spectroscopy: new methods and applications. Springer Science & Business Media; 2012.

[40] Romani A, Clementi C, Miliani C, Favaro G. Fluorescence spectroscopy: a powerful technique for the noninvasive characterization of artwork. JAoCR 2010;43(6):837—46. https://doi.org/10.1021/ar900291y.

[41] Itoh TJCr. Fluorescence and phosphorescence from higher excited states of organic molecules. 2012. p. 4541—68. https:// doi.org/10.1021/cr200166m. 112(8).

[42] Feng G, Zhang G-Q, Ding DJ. Design of superior phototheranostic agents guided by Jablonski diagrams. CSR 2020; 49(22):8179—234. https://doi.org/10.1039/d0cs00671h.

[43] Pascua JAA, Prado AJA, Solis BRB, Cid-Andres AP, Cjb Cambiador. Trends in fabrication, data gathering, validation, and application of molecular fluorometer and spectrofluorometer. JSAPAM Spectrosc B 2019;220:116837. https://doi.org/10.1016/j.saa.2019.02.061.

[44] DeRose PC, DeRose PC. Standard guide to fluorescence: instrument calibration and validation: US Department of Commerce, Technology Admininstration, National Institute of …. 2007. https://doi.org/10.6028/NIST.IR.7458.

[45] Uhl R. Arc lamps and monochromators for fluorescence microscopy. JCSHP 2012;2012(9). pdb. top071001, 10.1101/ pdb.top071001.

[46] Di Napoli T, Bujjamer JM, Illescas M, Barja B, Grecco HE. Giving new life to an outdated spectrofluorometer for static and time-resolved UCNP optical characterization. JNA 2025; 7(13):4214—20. https://doi.org/10.1039/d5na00330j.

[47] Putelli C. Design and development of a filterless spectrofluorometer. 2020. https://hdl.handle.net/10589/175472.

[48] Sudewi S, Sai Sashank PV, Kamaraj R, Zulfajri M, Huang GG. Understanding antibiotic detection with fluorescence quantum dots: A Review. JJoF 2025;35(5):2527—51. https://doi.org/10.1007/s10895-024-03743-4.

[49] Niu L-Y, Chen Y-Z, Zheng H-R, Wu L-Z, Tung C-H, Yang Q-Z. Design strategies of fluorescent probes for selective detection among biothiols. JCSR 2015;44(17):6143—60. https://doi.org/10.1039/c5cs00152h.

[50] Manning HB, Kennedy GT, Owen DM, Grant DM, Magee AI, Neil MA, et al. A compact, multidimensional spectrofluorometer exploiting supercontinuum generation. 2008. p. 494—505. https://doi.org/10.1002/jbio.200810051. 1(6).

[51] Kvittingen EV, Kvittingen L, Melø TB, Sjursnes BJ, Verley Rj. Demonstrating basic properties of spectroscopy using a self-constructed combined fluorimeter and UV-photometer. JoCE 2017;94(10):1486—91. https://doi.org/10.1021/acs.jchemed. 7b00121.

[52] Valeur B, Berberan-Santos MN. Molecular fluorescence: principles and applications. John Wiley & Sons; 2013.

[53] Jameson DM. Introduction to fluorescence. CRC Press; 2025.

[54] Demchenko AP. Fluorescence detection techniques. Introduction to fluorescence sensing. Springer; 2015. p. 69—132.

[55] Demchenko AP. Introduction to fluorescence sensing: volume 2: target recognition and imaging. Springer Nature; 2023.

[56] Eftink MR. Fluorescence quenching: theory and applications. Topics in fluorescence spectroscopy: principles. Springer; 2002. p. 53—126. https://doi.org/10.1007/978- 3-031-19089-6.

[57] Crisalli P, Kool ET. Multi-path quenchers: efficient quenching of common fluorophores. JBC 2011;22(11): 2345—54. https://doi.org/10.1021/bc200424r.

[58] Dobretsov G, Syrejschikova T, Smolina N. On mechanisms of fluorescence quenching by water. JB 2014;59(2):183—8. https://doi.org/10.1134/S0006350914020079.

[59] Dadras Moghaddam H, Khani R, Molaabasi F. Unique dual- functional and label-free turn-on and turn-off fluorescence biosensor to antibiotics using hemoglobin-capped platinum nanoribbons: from reduction-induced emission to inner filter effect and aggregation-caused Quenching. ACS Appl Bio Mater 2026;9(12):5137—50. https://doi.org/10.1021/acsabm. 6c00219.

[60] Serag A, Abdelazim AH, Ramzy S, Alnemari RM, Alzhrani RM, Abduljabbar MH, et al. A fluorescent sensor based on nitrogen-doped graphene quantum dots and molecularly imprinted polymers for selective and sensitive detection of meropenem in environmental and clinical matrices. Talanta 2026;296:128442. https://doi.org/10.1016/j. talanta.2025.128442.

[61] Baranova AA, Tyurin AP, Korshun VA, Alferova VA. Sensing of antibiotic—bacteria interactions. Antibiotics 2023; 12(8):1340. https://doi.org/10.3390/antibiotics12081340.

[62] Xing Y, Shen S, Wang Z. Modulating the intrinsic fluorescence properties of amoxicillin by sulfur nanodot-based nanoreactor for its direct fluorescence detection. JCEJ 2024; 499:156268. https://doi.org/10.1016/j.cej.2024.156268.

[63] Luo W, Ang CY. Determination of amoxicillin residues in animal tissues by solid-phase extraction and liquid chromatography with fluorescence detection. JJoAI 2000;83(1): 20—5. https://doi.org/10.1093/jaoac/83.1.20.

[64] Brittain HGJAP. Solid-state fluorescence of the trihydrate phases of ampicillin and amoxicillin. 2005. p. 55. https://doi. org/10.1208/pt060355. 6(3).

[65] García-Reiriz A, Damiani PC, AC Olivieri. Analysis of amoxicillin in human urine by photo-activated generation of fluorescence excitation—emission matrices and artificial neural networks combined with residual bilinearization. JACA 2007;588(2):192—9. https://doi.org/10.1016/j.aca.2007. 02.020.

[66] Saini A, Singh J, Kaur R, Singh N, Kaur N. Fluoremetric determination of amoxicillin drug in aqueous medium using hybrid framework of organic—inorganic nanoparticles. JS Chemical AB 2015;209:524—9. https://doi.org/10. 1016/j.snb.2014.12.018.

[67] Pawar SP, Walekar LS, Gunjal DB, Dalavi DK, Gore AH, Anbhule PV, et al. Fluorescence-based sensor for selective and sensitive detection of amoxicillin (Amox) in aqueous medium: Application to pharmaceutical and biomedical analysis. 2017. p. 918—23. https://doi.org/10.1002/bio.3271. 32(6).

[68] Shah K, Hassan E, Ahmed F, Anis I, Rabnawaz M, Shah MR, et al. Novel fluorene-based supramolecular sensor for selective detection of amoxicillin in water and blood. JE 2017; 141:25—9. https://doi.org/10.1016/j.ecoenv.2017.03.003.

[69] Zhang X, Ren Y, Ji Z, Fan JJ. Sensitive detection of amoxicillin in aqueous solution with novel fluorescent probes containing boron-doped carbon quantum dots. JoML 2020; 311:113278. https://doi.org/10.1016/j.molliq.2020.113278.

[70] Muthukumar A, Kalaiyar SJ. Fluorescence entrenched probe for onsite detection of amoxicillin residue in bovine milk. JoF 2024:1—12. https://doi.org/10.1007/s10895-024- 03959-4.

[71] Zhang L, Liu B, Li Z, Guo YJ. Study on interaction between cefixime and bovine serum albumin by modified fluorescence spectroscopy. AJoC 2015;27(4). https://doi.org/10. 14233/ajchem.2015.18289.

[72] Wang K, Dong Y, Bai X, Zhao X, Zhao R, Zhou J, et al. A water-stable Zn (II) coordination polymer as a fluorescence sensor for multifunctional detection of Cefixime in milk, honey, beef and chicken1285; 2023. p. 135495. https://doi. org/10.1016/j.molstruc.2023.135495.

[73] Bukhari N, Al-Warthan AA, Wabaidur SM, Othman ZA, Javid M, Haider S. Spectrofluorimetric determination of cefixime in pharmaceutical preparation and biological fluids using calcein as a fluorescence probe. JSL 2010;8(2):280—4. https://doi.org/10.1166/sl.2010.1264. [parative studies on the interaction of cefixime with bovine serum albumin by fluorescence quenching spectroscopy and synchronous fluorescence spectroscopy. JL 2015;30(5):686—92. https://doi. org/10.1002/bio.2805.

75] Akhgari F, Samadi N, KJ Farhadi. Fluorescent carbon dot as nanosensor for sensitive and selective detection of cefixime based on inner filter effect. JOF 2017;27(3):921—7. https://doi. org/10.1007/s10895-017-2027-0.

76] Zheng Y, Yang H, Yang Q, Li Y, Karmaker PG, Yang X, et al. A water-stable terbium-based MOF fluorescence sensor for rapid and highly sensitive quantitative detection of cefixime. JD 2024;230:112340. https://doi.org/10.1016/j.dyepig. 2024.112340.

[77] Yang CZ, Liu YC, Xu C, Bai AM, Hu YJ. A sensitive fluorescent sensor based on the photoinduced electron transfer mechanism for cefixime and ctDNA. JJoMR 2020;33(3):e2816. https://doi.org/10.1002/jmr.2816.

[78] Khan MN, Irum n, Mursaleen M. Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y. JL 2021; 36(2):515—24. https://doi.org/10.1002/bio.3971.

[79] Zhang J, Wang Z, Mi T, Wenren L, Wen K. A homogeneous fluorescence polarization immunoassay for the determination of cephalexin and cefadroxil in milk. JFam 2014;7(4): 879—86. https://doi.org/10.1007/s12161-013-9695-4.

[80] Bian S, Chu X, Jin Y, Xing S, Zhang Y, Hu H. A novel microsphere-based fluorescence immunochromatographic assay for monitoring cefalexin residues in plasma, milk, muscle and liver. JAM 2013;5(22):6441—8. https://doi.org/10. 1039/c3ay41487f.

[81] Hao A-Y, Wang X-Q, Mei Y-Z, Nie J-F, Yang Y-Q, Dai CC, et al. A smartphone-combined ratiometric fluorescence probe for specifically and visibly detecting cephalexin. JSAPAM 2021;249:119310. https://doi.org/10.1016/j.saa.2020. 119310.

[82] Jalili R, Irani-Nezhad MH, Khataee A, Joo SW. A ratiometric fluorescent probe based on carbon dots and gold nanocluster encapsulated metal—organic framework for detection of cephalexin residues in milk. JSAPAM Spectros B 2021;262:120089. https://doi.org/10.1016/j.saa.2021.120089.

[83] Chullasat K, Kanatharana P, Bunkoed O. Nanocomposite optosensor of dual quantum dot fluorescence probes for simultaneous detection of cephalexin and ceftriaxone. JS Chem AB 2019;281:689—97. https://doi.org/10.1016/j.snb. 2018.11.003.

[84] Saikia D, Dutta P, Sarma NS, Adhikary NC. CdTe/ZnS core/ shell quantum dot-based ultrasensitive PET sensor for selective detection of Hg (II) in aqueous media. JS Chem AB 2016;230:149—56. https://doi.org/10.1016/j.snb.2016.02.035.

[85] Meng X, Peng J. Liquid chromatographic analysis of Cephalexin in human plasma by fluorescence detection of the 9-fluorenylmethyl chloroformate derivative. JAl 2009; 42(12):1844—54. https://doi.org/10.1080/00032710903060743.

[86] Li L, Zhang Q, Ding Y, Cai X, Gu S, Cao Z. Application of l- cysteine capped core—shell CdTe/ZnS nanoparticles as a fluorescence probe for cephalexin. JAM 2014;6(8):2715—21. https://doi.org/10.1039/c4ay00094c.

[87] Khorsheed KM, Tahir TF. Fluorescent [Nd (Anth) 3 (H 2 O) 3 ] Complex for determination of some β-lactam antibiotics. Acad J Inter Univ Erbil 2026;3(1):789—810. https://orcid.org/ 0000-0001-9298-4569.

[88] Korytkowski MJ, Baraniak A, Boryło A, Rudnicki- Velasquez P. Luminescence-Based Strategies for Detecting β-Lactamase activity: a review of the last decade. JL 2026; 16(2):250. https://doi.org/10.3390/life16020250.

[89] Kumar D, Singh H, Bhardwaj N, Soni S, Deep A. Fluorescent nanomaterials for early detection of cancer biomarkers: a review. JMA 2025;192(12):837. https://doi.org/10.1007/ s00604-025-07679-9.

[90] Pauter K, Szultka-Młynska M, Buszewski B. Determination and identification of antibiotic drugs and bacterial strains in biological samples. Molecules 2020;25(11):2556. https://doi. org/10.3390/molecules25112556.

[91] Choucair I. Chromatography and mass spectrometry in therapeutic drug monitoring: Method performance, practical trade-offs, and implementation considerations. J Chromatogr B 2026:125022. https://doi.org/10.1016/j. jchromb.2026.125022.

[92] Verdier M-C, Tribut O, Tattevin P, Le Tulzo Y, Michelet C, Bentue-Ferrer D. Simultaneous determination of 12 β-lactam antibiotics in human plasma by high-performance liquid chromatography with UV detection: application to therapeutic drug monitoring. Antimicrob Agents Chemother 2011;55(10):4873—9. https://doi.org/10.1128/aac.00533- 11.

[93] Chen L, Wang B, Diao Z, Zhao M, Xie K, Zhang P, et al. Development and validation of an HPLC-ESI/MS/MS method for the determination of amoxicillin, its major metabolites, and ampicillin residues in chicken tissues. Molecules 2019;24(14):2652. molecules24142652. https://doi.org/10.3390/

[94] Jank L, Martins MT, Arsand JB, Hoff RB, Barreto F, Pizzolato TM. High-throughput method for the determination of residues of β-lactam antibiotics in bovine milk by LC-MS/MS. Food Addit Contam 2015;32(12):1992—2001. https://doi.org/10.1080/19440049.2015.1099745.

[95] Zhong W. Nanomaterials in fluorescence-based biosensing. Anal Bioanal Chem 2009;394(1):47—59. https://doi.org/10. 1007/s00216-009-2643-x.

[96] Song S, Han L, Chen M, Pan L, Tu K. Recent progress in nanomaterial-based fluorescence assays for the detection of food-borne pathogens. Sensors 2024;24(23):7715. https://doi. org/10.3390/s24237715.

[97] Harshita Wu HF, Kailasa SK. Recent advances in nanomaterials-based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules). JL 2023;38(7):954—98. https://doi.org/10.1002/bio.4353.

[98] Demchenko AP. The concept of λ-ratiometry in fluorescence sensing and imaging. JJOF 2010;20(5):1099—128. https://doi. org/10.1007/s10895-010-0644-y.

[99] Ljubojevic Pelic D, Radosavljevic V, Pelic M,  Zivkov Balo s M, Puvaca N, Jug-Dujakovic J, et al. Antibiotic residues in cultured fish: implications for food safety and regulatory concerns. 2024. p. 484. https://doi.org/10.3390/ fishes9120484. 9(12).