Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates

Authors

Hawkar S. Omer, Department of Pharmacognosy, College of Pharmacy, Hawler Medical University
Safaa T. Aka, Department of Pharmacognosy, College of Pharmacy, Hawler Medical University

Corresponding Author

Hawkar S. Ome

Authors ORCID

0000-0003-4909-0599

Document Type

Research Article

Abstract

Multi-drug resistant microorganisms have caused a remarkable increase in hospital-acquired infections (HAI)during the previous few decades. Moreover, microbiological contamination of antiseptics and disinfectants has been recorded in medical settings.

Keywords

Acinetobacter baumannii, Chlorhexidine, Biofilm, Multiresistant.

How to Cite This Article

Omer, Hawkar S. and Aka, Safaa T. (2023) "Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates," Polytechnic Journal: Vol. 12: Iss. 2, Article 9.
DOI: https://doi.org/10.25156/ptj.v12n2y2022.pp85-91

References

A'SHIMI, M. H. N., ALATTRAQCHI, A. G., RANI, F. M., RAHMAN, N. I. A., ISMAIL, S., ABDULLAH, F. H., OTHMAN, N., CLEARY, D. W., CLARKE, S. C. & YEO, C. C. 2019. Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia. The Journal of Infection in Developing Countries, 13(07), 626-633.

AKHLAGHI, N., SADEGHI, M., FAZELI, F., AKHLAGHI, S., MEHNATI, M. & SADEGHI, M. 2019. The antibacterial effects of coffee extract, chlorhexidine, and fluoride against Streptococcus mutans and Lactobacillus plantarum: An in vitro study. Dental research journal, 16(5), 346-353.

AL-GHAZALY, N. & TUWAIJ, N. 2022. Distribution of AmpC-Family genes among Acinetobacter spp isolates in Najaf City, Iraq. International journal of health sciences, 6(S1), 10152– 10161

AMSALU, A., SAPULA, S. A., DE BARROS LOPES, M., HART, B. J., NGUYEN, A. H., DRIGO, B., TURNIDGE, J., LEONG, L. E. & VENTER, H. 2020. Efflux pump-driven antibiotic and biocide crossresistance in Pseudomonas aeruginosa isolated from different ecological niches: A case study in the development of multidrug resistance in environmental hotspots. Microorganisms, 8(11), 1647.

APISARNTHANARAK, A., HSU, L. Y., LIM, T.-P. & MUNDY, L. M. 2014. Increase in chlorhexidine minimal inhibitory concentration of Acinetobacter baumannii clinical isolates after implementation of advanced source control. Infection Control & Hospital Epidemiology, 35(1), 98-99.

AZIZ, R. A. R. & AL-JUBORI, S. S. 2017. Molecular analysis of genetic elements responsible for XDR in highly successful pathogen Acinetobacter baumannii isolated from clinical samples of Iraqi patients. J Glob Pharma Technol, 9(4), 26-39.

BALAURE, P. C. & GRUMEZESCU, A. M. 2020. Recent advances in surface nanoengineering for biofilm prevention and control. Part II: active, combined active and passive, and smart bacteria-responsive antibiofilm nanocoatings. Nanomaterials, 10(6), 1527.

BISWAS, D., TIWARI, M. & TIWARI, V. 2019. Molecular mechanism of antimicrobial activity of chlorhexidine against carbapenemresistant Acinetobacter baumannii. PloS one, 14(10), e0224107.

CARUSO, C., PORTA, A., TOSCO, A., ELETTO, D., PACENTE, L., BARTOLLINO, S. & COSTAGLIOLA, C. 2020. A Novel vitamin E TPGS-based formulation enhances chlorhexidine bioavailability in corneal layers. Pharmaceutics, 12(7), 642.

CHIANG, S.-R., JUNG, F., TANG, H.-J., CHEN, C.-H., CHEN, C.-C., CHOU, H.-Y. & CHUANG, Y.-C. 2018. Desiccation and ethanol resistances of multidrug resistant Acinetobacter baumannii embedded in biofilm: The favorable antiseptic efficacy of combination chlorhexidine gluconate and ethanol. Journal of Microbiology, Immunology and Infection, 51(6), 770-777

CIEPLIK, F., JAKUBOVICS, N. S., BUCHALLA, W., MAISCH, T., HELLWIG, E. & AL-AHMAD, A. 2019. Resistance toward chlorhexidine in oral bacteria–is there cause for concern? Frontiers in microbiology, 10, 587.

CLINICAL & INSTITUTE, L. S. 2015. M100-S25 Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI, 35, 1-240

FUANGTHONG, M., JULOTOK, M., CHINTANA, W., KUHN, K., RITTIROONGRAD, S., VATTANAVIBOON, P. & MONGKOLSUK, S. 2011. Exposure of Acinetobacter baylyi ADP1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself and to oxidants. Journal of Antimicrobial Chemotherapy, 66(2), 319-322.

HASHEMI, M. M., HOLDEN, B. S., COBURN, J., TAYLOR, M. F., WEBER, S., HILTON, B., ZAUGG, A. L., MCEWAN, C., CARSON, R. & ANDERSEN, J. L. 2019. Proteomic analysis of resistance of Gram-negative bacteria to chlorhexidine and impacts on susceptibility to colistin, antimicrobial peptides, and ceragenins. Frontiers in microbiology, 10, 210.

HOUARI, A. & DI MARTINO, P. 2007. Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation. Letters in applied microbiology, 45(6), 652-656.

JAIN, N., JANSONE, I., OBIDENOVA, T., SIMANIS, R., MEISTERS, J., STRAUPMANE, D. & REINIS, A. 2021. Antimicrobial Resistance in Nosocomial Isolates of Gram-Negative Bacteria: Public Health Implications in the Latvian Context. Antibiotics, 10(7), 791.

KING, L. B., SWIATLO, E., SWIATLO, A. & MCDANIEL, L. S. 2009. Serum resistance and biofilm formation in clinical isolates of Acinetobacter baumannii. FEMS Immunology & Medical Microbiology, 55(3), 414-421.

LIU, W. J., FU, L., HUANG, M., ZHANG, J. P., WU, Y., ZHOU, Y. S., ZENG, J. & WANG, G. X. 2017. Frequency of antiseptic resistance genes and reduced susceptibility to biocides in carbapenem-resistant Acinetobacter baumannii. Journal of medical microbiology, 66(1), 13-17.

MAGIORAKOS, A.-P., SRINIVASAN, A., CAREY, R. B., CARMELI, Y., FALAGAS, M., GISKE, C., HARBARTH, S., HINDLER, J., KAHLMETER, G. & OLSSON-LILJEQUIST, B. 2012. Multidrugresistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection, 18(3), 268- 281.

NATH, S., SINHA, A., SINGHA, Y. S., DEY, A., BHATTACHARJEE, N. & DEB, B. 2020. Prevalence of antibiotic-resistant, toxic metaltolerant and biofilm-forming bacteria in hospital surroundings. Environmental Analysis, Health and Toxicology, 35(3):e2020018.

NOWAK, J., ZANDER, E., STEFANIK, D., HIGGINS, P. G., ROCA, I., VILA, J., MCCONNELL, M. J., CISNEROS, J. M., SEIFERT, H. & WP4, M. W. G. 2017. High incidence of pandrugresistant Acinetobacter baumannii isolates collected from patients with ventilator-associated pneumonia in Greece, Italy and Spain as part of the MagicBullet clinical trial. Journal of Antimicrobial Chemotherapy, 72(12), 3277-3282.

ORTEGA‐PEÑA, S., HIDALGO‐GONZÁLEZ, C., ROBSON, M. C. & KRÖTZSCH, E. 2017. In vitro microbicidal, anti‐biofilm and cytotoxic effects of different commercial antiseptics. International Wound Journal, 14(3), 470-479.

RAJAMOHAN, G., SRINIVASAN, V. & GEBREYES, W. A. 2009. Biocide-tolerant multidrug-resistant Acinetobacter baumannii clinical strains are associated with higher biofilm formation. Journal of Hospital Infection, 73(3), 287-289.

RANGEL, K., CHAGAS, T. P. G. & DE-SIMONE, S. G. 2021. Acinetobacter baumannii infections in times of COVID-19 pandemic. Pathogens, 10, 1006.

RÖHNER, E., JACOB, B., BÖHLE, S., ROHE, S., LÖFFLER, B., MATZIOLIS, G. & ZIPPELIUS, T. 2020. Sodium hypochlorite is more effective than chlorhexidine for eradication of bacterial biofilm of staphylococci and Pseudomonas aeruginosa. Knee Surgery, Sports Traumatology, Arthroscopy, 28(12), 3912-3918.

SALEHI, B., GOUDARZI, H., NIKMANESH, B., HOURI, H., ALAVIMOGHADDAM, M. & GHALAVAND, Z. 2018. Emergence and characterization of nosocomial multidrug-resistant and extensively drug-resistant Acinetobacter baumannii isolates in Tehran, Iran. Journal of infection and chemotherapy, 24(7), 515-523.

SEHREE, M. M., ABDULLAH, H. N. & JASIM, A. M. 2021. Isolation and Evaluation of Clinically Important Acinetobacter Baumannii From Intensive Care Unit Samples. Journal of Techniques, 3(3), 83- 90.

ŞIMŞEK, M. & DEMIR, C. 2020. Determination of colistin and tigecycline resistance profile of acinetobacter baumannii strains from different clinical samples in a territory hospital in Turkey. Journal of Health Science and Medical Research, 38(2), 81-91.

SMAI, S. B. 2020. A Prevalence of infections with antibiotic-resistant Acinetobacter baumannii in different clinical samples from hospitals in Erbil. Zanco Journal of Pure and Applied Sciences, 32(3), 95-100.

SOBOUTI, B., MIRSHEKAR, M., FALLAH, S., TABAEI, A., MEHRABADI, J. F. & DARBANDI, A. 2020. Pan drug-resistant Acinetobacter baumannii causing nosocomial infections among burnt children. Medical journal of the Islamic Republic of Iran, 34, 24.

THEURETZBACHER, U. 2017. Global antimicrobial resistance in Gram-negative pathogens and clinical need. Current opinion in microbiology, 39, 106-112.

TOKAJUK, G., NIEMIROWICZ, K., DEPTUŁA, P., PIKTEL, E., CIEŚLUK, M., WILCZEWSKA, A. Z., DĄBROWSKI, J. R. & BUCKI, R. 2017. Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity. International journal of nanomedicine, 12, 7833.

Publication Date

2-1-2023