Vitamin D3 is a potent antiproliferative agent against various tumor cells in vitro. Here, the results of Vitamin D3 study as a potential antitumor therapy in vitro are presented. Applying antiproliferative 3(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2H-terazolium bromide assays, the inhibitory effects of the Vitamin D were measured. The following cancer cell lines were employed: L20B (normal cell line) and RD (malignant rhabdomyosarcoma). Both cell lines were cultivated in 96-wells culture plates in the presence and absence of different doses of Vitamin D (10–6, 10–8, and 10–10 ?g/ml) for 24 and 48 h. In vitro results of cytotoxic effects were variable on both cell lines, according to dose and exposure time, after 24 h exposure of RD, the highest concentration of Vitamin D3(10?6 ?g/ml) treatment had significant effect in decreasing cell proliferation from O.D (0.4570 ± 0.0302) to (0.1540 ± 0.0017) as compared with negative control, with increasing concentrations the cytotoxicity is increased directly proportional; thus, the lowest cytotoxic effect was at the lowest concentration of both Vitamin D3 (10?12 ?g/ml). While after 48 h, the same concentration of Vitamin D3 shows an increase in proliferation from 0.3710 ± 0.0023 to 0.4597 ± 0.0017 on the RD cell line. While a significant increase in L20B cell proliferation was observed after 24 h treatment at the concentration (10?6 ?g/ml) from 0.3570 ± 0.0011 to 0.0330 ± 0.0017, when compared with the negative control. However, after 48 h treatment, a significant increases the proliferation of cells as shown from O.D 0.2927 ± 0.0008 to 0.4300 ± 0.0011, respectively. Thus, the present study was aimed to evaluate the antiproliferative property of Vitamin D and its relation to inhibition of cancer cell growth
3(4, 5-dimethyl- 2-thiazolyl)-2, 5-diphenyl-2H-terazolium bromide, Tumor cell line, Vitamin D3, RD, L20B
How to Cite This Article
Mustafa, Twana A. and Rasul, Iman M.
"Cytotoxic Effects of Vitamin D3 on Tumor Cell Lines,"
Polytechnic Journal: Vol. 9:
2, Article 17.