Document Type
Research Article
Abstract
The comparison of histopathological and biochemical effects of L-cysteine, silymarin and ursodeoxycholic acid which have a protective effect on kidney function against CCl4 induce nephrotoxicity were investigated in this study. Twenty adult male rats were divided into 5 groups and treated as follows, the control group was administrated 1.5 ml/kg. B.W physiological solution (0.9%) orally, Carbon Tetrachloride group (CCl4 1.5 ml/kg. B.W), L-cysteine (100mg/kg B.W.) +CCl4 group, ursodeoxycholic acid (50 ml/kg B.W.) +CCl4 group, and the last group was Silymarin 100 mg/kg. B.W with CCl4, the experiment lasted 30 days. Oxidative stress caused by CCl4 caused increasing in Creatinine and Urea levels in serum while decreasing the level of uric acid and albumin when compared with the control group, L-cysteine, Silymarin and the drug presented ameliorating effect by decreasing creatinine, Urea, and increasing the level of uric acid with albumin. Histopathological results showed that CCl4 caused oxidative damage in kidney tubules in comparison with control group, while Silymarin, L-cysteine showed nephroprotective effects
Keywords
L-cysteine, Silymarin, CCl4, Kidney, Oxidative stress, Rats
How to Cite This Article
Saed, Hero H. Muhammed Mrs.; Jafar, Sarwar N. Mr.; Ali, Zeyan A. Mrs.; and Ismail, Treefa F. Mrs.
(2023)
"Nephroprotective Roles of L-Cysteine, Silymarin and Ursodeoxycholic acid, against Carbon Tetrachloride induced Nephrotoxicity in Male Albino Rats,"
Polytechnic Journal: Vol. 13:
Iss.
1, Article 15.
DOI: https://doi.org/10.59341/2707-7799.1739
References
ADEWOLE, S., SALAKO, A., DOHERTY, O. & NAICKER, T. 2007. Effect of melatonin on carbon tetrachloride-induced kidney injury in Wistar rats. African Journal of Biomedical Research, 10.
AHMED, M. A., TAYAWI, H. M. & IBRAHIM, M. K. 2019. Protective effect of Silymarin against kidney injury induced by carbon tetrachloride in male rats. Iraqi Journal of Veterinary Sciences, 33, 127-130.
AHMED, S. F. 2016. Evaluation of the protective effect of nebivolol against gentamicin-induced nephrotoxicity in rats. Curr. Sci. Int, 5, 205-214.
AL-SALMI, F. A., AL-EISA, R., HAMZA, R. Z., KHALED, H. E. & EL-SHENAWY, N. S. 2019. Protective Effect of L-cysteine Against Sodium Valproate-induced Oxidant Injury in Testis of Rats. International Journal of Pharmacology, 15, 248-256.
BONIFÁCIO, V. D., PEREIRA, S. A., SERPA, J. & VICENTE, J. B. 2021. Cysteine metabolic circuitries: Druggable targets in cancer. British journal of cancer, 124, 862-879.
BRIGUORI, C., QUINTAVALLE, C., DE MICCO, F. & CONDORELLI, G. 2011. Nephrotoxicity of contrast media and protective effects of acetylcysteine. Archives of toxicology, 85, 165-173.
CHANEY, A. L. & MARBACH, E. P. 1962. Modified reagents for determination of urea and ammonia. Clinical chemistry, 8, 130-132.
CLEMENTE PLAZA, N., REIG GARCÍA-GALBIS, M. & MARTÍNEZ-ESPINOSA, R. M. 2018. Effects of the Usage of l-Cysteine (l-Cys) on Human Health. Molecules, 23, 575.
DOUMAS, B. T., WATSON, W. A. & BIGGS, H. G. 1997. Albumin standards and the measurement of serum albumin with bromcresol green. Clinica chimica acta, 258, 21-30.
FARARH, K. M., FARID, A. S., AZZAM, I. M. & SULTAN, A. A. 2016. The hepato-protective Effect of Stem Cells and Levamisole against Carbontetrachloride induced Liver Fibrosis. Benha Veterinary Medical Journal, 31, 149-157.
FOSSATI, P., PRENCIPE, L. & BERTI, G. 1980. Use of 3, 5-dichloro-2-hydroxybenzenesulfonic acid/4- aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clinical chemistry, 26, 227-231.
GOUNDEN, V., VASHISHT, R. & JIALAL, I. 2018. Hypoalbuminemia.
HAMZA, R. & EL-SHENAWY, N. 2017. The beneficial effects of l-cysteine on brain antioxidants of rats affected by sodium valproate. Human & Experimental Toxicology, 36, 1212-1221.
HAMZA, R. Z., ISMAIL, H. A., KHALED, H. E. & ELSHENAWY, N. S. 2015. Antioxidant effect of Lcysteine on sodium-valproate-induced oxidative stress in rat liver: biochemical and ultrastructural approaches. Toxicological & Environmental Chemistry, 97, 1041-1056.
HARMS, M. H., VAN BUUREN, H. R., CORPECHOT, C., THORBURN, D., JANSSEN, H. L., LINDOR, K. D., HIRSCHFIELD, G. M., PARÉS, A., FLOREANI, A. & MAYO, M. J. 2019. Ursodeoxycholic acid therapy and liver transplantfree survival in patients with primary biliary cholangitis. Journal of Hepatology, 71, 357-365.
HISMIOGULLARI, A. A., HISMIOGULLARI, S. E., KARACA, O., SUNAY, F. B., PAKSOY, S., CAN, M., KUS, I., SEYREK, K. & YAVUZ, O. 2015. The protective effect of curcumin administration on carbon tetrachloride (CCl 4)-induced nephrotoxicity in rats. Pharmacological Reports, 67, 410-416.
HSU, C.-N., HOU, C.-Y., CHANG-CHIEN, G.-P., LIN, S. & TAIN, Y.-L. 2022. Dietary Supplementation with Cysteine during Pregnancy Rescues Maternal Chronic Kidney Disease-Induced Hypertension in Male Rat Offspring: The Impact of Hydrogen Sulfide and Microbiota-Derived Tryptophan Metabolites. Antioxidants, 11, 483.
JACOBS, A. T. & MARNETT, L. J. 2010. Systems analysis of protein modification and cellular responses induced by electrophile stress. Accounts of chemical research, 43, 673-683.
KHAZAEI, R., SEIDAVI, A. & BOUYEH, M. 2021. A review on the mechanisms of the effect of silymarin in milk thistle (Silybum marianum) on some laboratory animals. Veterinary Medicine and Science.
LARSEN, K. 1972. Creatinine assay by a reaction-kinetic principle. Clin Chim Acta, 41, 209-17.\
MACDONALD-RAMOS, K., MICHÁN, L., MARTÍNEZ-IBARRA, A. & CERBÓN, M. 2020. Silymarin is an ally against insulin resistance: A review. Annals of Hepatology.
MAZANI, M., REZAGHOLIZADEH, L., SHAMSI, S., MAHDAVIFARD, S., OJARUDI, M., SALIMNEJAD, R. & SALIMI, A. 2022. Protection of CCl4-induced hepatic and renal damage by linalool. Drug and Chemical Toxicology, 45, 963- 971.
MOHAN, M. S. G., RAMAKRISHNAN, T., MANI, V. & ACHARY, A. 2018. Protective effect of crude sulphated polysaccharide from Turbinaria ornata on isoniazid rifampicin induced hepatotoxicity and oxidative stress in the liver, kidney and brain of adult Swiss albino rats.
MUKAI, T., MERA, K., NISHIDA, K., NAKASHIMA, M., SASAKI, H., SAKAEDA, T. & NAKAMURA, J. 2002. A novel method for preparation of animal models of liver damage: liver targeting of carbon tetrachloride in rats. Biological and Pharmaceutical Bulletin, 25, 1494-1497.
OGETURK, M., KUS, I., KAVAKLI, A., ONER, J., KUKNER, A. & SARSILMAZ, M. 2005. Reduction of carbon tetrachloride‐ induced nephropathy by melatonin administration. Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease, 23, 85-92.
PISTE, P. 2013. Cysteine–master antioxidant. International journal of pharmaceutical, chemical and biological sciences, 3, 143-149.
SALMAN, M. & RANDA, R. A. 2016. Pathophysiological studies on the Reverse Effect of Curcumin (curcuma longa, Zingiberaceae) and Ursofalk (Ursodeoxycholic acid) against the Toxicity of Carbon Tetrachloride on Albino Rats. J Liver, 5, 2167-0889.1000200.
URQUHART, B. L. & NOLIN, T. D. 2020. Drug metabolism in chronic kidney disease. Chronic Renal Disease. Elsevier.
VARGAS-MENDOZA, N., MADRIGALSANTILLÁN, E., MORALES-GONZÁLEZ, Á., ESQUIVEL-SOTO, J., ESQUIVEL-CHIRINO, C., Y GONZÁLEZ-RUBIO, M. G.-L., GAYOSSO-DELUCIO, J. A. & MORALES-GONZÁLEZ, J. A. 2014. Hepatoprotective effect of silymarin. World journal of hepatology, 6, 144.
VENKATANARAYANA, G., SUDHAKARA, G., SIVAJYOTHI, P. & INDIRA, P. 2012. Protective effects of curcumin and vitamin E on carbon tetrachloride-induced nephrotoxicity in rats. EXCLI journal, 11, 641.
YOSHIOKA, H., USUDA, H., FUKUISHI, N., NONOGAKI, T. & ONOSAKA, S. 2016. Carbon tetrachloride-induced nephrotoxicity in mice is prevented by pretreatment with zinc sulfate. Biological and pharmaceutical Bulletin, 39, 1042- 1046
Publication Date
9-1-2023
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