Prevalence of Rotavirus and Adenovirus Infections among Children under 5 Years Old with Gastroenteritis Admitted To Raparin Pediatric Hospital, Erbil Province

Authors

Karim Jwan Saleh khoshnaw, Nursing Department/ Erbil Medical Technical Institute, Erbil Polytechnic University
Hussein M. Abdullah, Nursing Department/ Erbil Medical Technical Institute, Erbil Polytechnic University
Pshtewan D. Majeed, Erbil Polytechnic University

How to Cite This Article

Saleh khoshnaw, Karim Jwan; Abdullah, Hussein M.; and Majeed, Pshtewan D. (2018) "Prevalence of Rotavirus and Adenovirus Infections among Children under 5 Years Old with Gastroenteritis Admitted To Raparin Pediatric Hospital, Erbil Province," Polytechnic Journal: Vol. 8: Iss. 2, Article 13.
DOI: https://doi.org/10.25156/ptj.2018.8.2.147

Document Type

Original Article

Abstract

Background: Gastroenteritis is considered as one of the leading causes of morbidity and mortality in children less than 5 years of age, especially in developing countries. Rotavirus and Adenovirus are recognized as common etiologies of this condition. Objectives: In Erbil province, little information are reported about these viral infections among children under 5 years old, so we investigated two of the most important agents, Rotavirus and Adenovirus to provide epidemiological evidence for a better understanding of their role among children with diarrhea. Methods: A total of 300 stool samples from pediatric patients (less than 5 years old) with gastroenteritis who were admitted to Raparin pediatric hospital, Erbil, Iraq, from May to October 2016 were collected and screened for presence of Rotavirus Group A (RovA) and Enteric Adenovirus type 40/41 (EAd40/41) antigens by using rapid immunochromatographic assay (Plasmatic Laboratory Products, DT6 5BU United Kingdom). Results: Out of the 300 samples examined, Rotavirus was detected in 65 samples (21.67%), Adenovirus was identified in 19 (6.33%) of patients and 12 (4%) of specimens had Rotavirus-Adenovirus co-infection. The findings showed that Rotavirus is significantly higher than Adenovirus. The Infections were high predominant among children old, with a maximum incidence among infants 1-12 months. The frequency of viral infection was higher in male 33.33% patients compared to females 30.3%. The viral infection in patients from rural areas (34.64%) was more frequent than those from urban areas (30.06%). The result revealed that the highly incidence were in infant using bottle-feed (36.55%) in comparing with infants who are breast-feed (28.20%) and mixed-feed (30.43%). No significant associations were observed with gender, residency and feeding types.

Publication Date

5-1-2018

References

1. Ali, S.H.M.; Al-Wadi, G.I.A.; Yassin, B.A.G.; Al-Janabi, M.K.W.; Al-Timimi, M.F. and Hussein, H.S. 2016. Prevalence of Rotavirus, adenovirus, astrovirus and enteroviruses Involved in acute infantile and childhood gastroenteritis in Baghdad: a Stool- based antigenic study. Inter. J. of Info. Res. Rev. 3(6):2467-2475.

2. Al Laham, N.; Elyazji, M.; Al-Haddad, R. and Ridwan, F. 2015. Prevalence of enteric pathogen-associated community gastroenteritis among kindergarten children in Gaza. J. Biome. Res. 29(1):61-68.

3. Badry, A.H.H.; Jameel, A.Y. and Mero, W.M.S. 2014. Pathogenic microorganisms associated with diarrhea in infants and children in Duhok province, Kurdistan region / Iraq. J. Unive. Zakho. 2A (2): 266-275.

4. Ali, S.H.M.; Al-Wadi, G.I.A.; Abu-Alees, H.K.M.; Mohammed, K.I.A.; Yassin, B.A.G.; AlTimimi, M.F. and Al-Janabi, M.K.W. 2016. Seasonal trending of Rotavirus infection in infantile patients from Baghdad with acute gastroenteritis. Pharma. Innov. J. 5(12): 37-44.

5. Al-Shuwaikh, A.M.A. 2016. Frequency of Rotavirus, Adenovirus and Astrovirus among Patients with Acute Diarrhea by Chromatographic Immunoassay and Enzyme Linked Immunosorbent Assay. J. Biotech. Res. Center. 10 (2):58-64.

6. Al-Sadoon, M.A. 2012. Rotaviral and protozoal infections among malignant children in Basrah Governorate. T.Q.M.J. 6(1):173-180.

7. Al-Khoweledy, A.J.Sh. 2017. Detection of Adenovirus Type 40/41 among children with diarrhea, In Al-Najaf province, Iraq. J. Babylon Univ./Pure and App. Sci. 3(25):1212-1218.

8. Uwimana1, J.; Ndishimye, P.; Bizimana, E.; Byiringiro, J. and Mutesa, L. 2015. Rotavirus gastroenteritis surveillance and prevalence assessment among under five children in Rwanda. R.M.J. 4(75):17-21.

9. Ozsari, T.; Bora, G.; Kaya, B. and Yakut, K. 2016. The Prevalence of Rotavirus and Adenovirus in the Childhood Gastroenteritis. Jundishapur J. Microbiol. 9(6):1-5.

10. Al-Badani, A.; Al-Areqi, L.; Majily, A.; AL-Sallami, S.; AL-Madhagi, A. and AL-Kamarany, M.A. 2014. Rotavirus Diarrhea among Children in Taiz, Yemen: Prevalence-Risk Factors and Detection of Genotypes. Inter. J. Ped. Article ID 928529: 1-10.

11. ALkheliaf, R.; Garout, M.A.; Abdelwadood, M.R.; Alkhlaif, A. and Khelaif, M. R. 2017. Rotavirus Infection in a Secondary Hospital, Laboratory Diagnosis and Impact of Immunization on Pediatric Hospitalization Saudi Arabia. American J. Epidemiol. Infect. Dis. 5(2):21-26.

12. Ribeiro, J.; Ferreira, D.; Arrabalde, C.; Almeida, S.; Baldaque, I. and Sousa, H. 2015. Prevalence of Adenovirus and Rotavirus infection in immunocompromised patients with acute gastroenteritis in Portugal. World J. Virol. 4(4): 372-376.

13. Elhag, W.I.; Saeed, H.A.; Omer, El. E. and A.S. Ali, 2013. Prevalence of Rotavirus and Adenovirus associated with diarrhea among displaced communities in Khartoum, Sudan. BMC Infect. Dis. 13(209):1-6.

14. Motamedifar, M.; Amini, E. and Shirazi, P.T. 2013. Frequency of Rotavirus and Adenovirus Gastroenteritis among children in Shiraz, Iran. Iranian Red Crescent Med.J. 15(8):729-733.

15. Al-Sayidi, R.H.E.; Fadhil, H.Y. and AL-Hamdani, F.G. 2014. Rapid Diagnosis of RotaAdenoviruses for Acute Gastroenteritis in hospitalized Children under 4 Years Old, Baghdad. Int. J. Curr. Microbiol. App. Sci. 3(1): 453-458.

16. Kajbaf, T.Z.; Shamsizadeh, A.; Kalvandi, G. and Macvandi, M. 2013. Relative Frequency of Rotavirus and Adenovirus among Children Aged 1-60 Months Hospitalized With Acute Diarrhoea in South-Western, Iran. Jundishapur J. Microbiol. 6(1): 47-50.

17. Akan, H.; İzbırak, G.; Gürol, Y.; Sarıkaya, S.; Gündüz, T.S.; Yılmaz, G.; Hayran, O. and Vitrinel, A. 2009. Rotavirus and Adenovirus frequency among patients with acute gastroenteritis and their relationship to clinical parameters: a retrospective study in Turkey. Asia Pac. Fam. Med. 8(8):1-8.

18. Jaff, D.O.; Aziz, T.A.G. and Smith, N.R. 2016. The Incidence of Rotavirus and Adenovirus Infections among Children with Diarrhea in Sulaimani Province, Iraq. J. Biosci. Med. 4:124- 131.

19. Shokrollahi, M.R.; Noorbakhsh, S.; Monavari, H.R.; Darestani, S.G.; Motlagh, A.V. and Nia, S.J. 2014. Acute Nonbacterial Gastroenteritis in Hospitalized Children: A Cross Sectional Study. Jundishapur J. Microbiol. 7(12): 1-5.

20. Celik, C., Gozel, M. G.; Turkay, H.; Bakici, M. Z.; Güven, A. S. and Elaldi, N. 2015. Rotavirus and Adenovirus gastroenteritis: time series analysis. Japan Ped. Soc. 57(4):590-596.

21. Hegazi, M.A.; Sayed, M.H.; Sindi, H.H.; Bekhit, O.E.; El-Deek, B.S.; Alshoudri, F.M.Y. and Noorelahi, A.K. 2017. Is Rotavirus still a major cause for diarrheal illness in hospitalized pediatric patients after Rotavirus vaccine introduction in the Saudi national immunization program? Medicine. 96(15):1-7.

22. Nafi, O. 2010. Rotavirus gastroenteritis among children aged under 5 years in Al Karak, Jordan. E.M.H.J. 16(10):1064-1069.

23. Al Musawi, M.; Zainaldeen, H.; Shafi, F.; Anis, S. and DeAntonio, R. 2013. Rotavirus gastroenteritis in children under 5 years in the Kingdom of Bahrain: hospital-based surveillance. Clin. Epidemiol. 5: 269-275

24. Zaman, N.A., Al-Tae, A.A., Saadoon, I.H. 2012. Prevalence of Rotavirus, Adenovirus, and Rotavirus Adenovirus Confection among children less than 5 years in Kirkuk city. 2 nd Scientific Conference-Science College-Tikrit University.

25. Hussein, A.M. and Hassan, M.K. 2000. Rotavirus infection among hospitalized children with acute watery diarrhea in Basrah-Iraq. Bahrain Med. Bulletin. 22(4):1-5.

26. Abed, L.M. and Al-kabi, S.J. 2016. Comparison between the Interleukin-6 levels in patients infected with Rotavirus and Adenovirus in AL-Najaf AL-Ashraf governorate. Al-Kufa Univ. J. Biol. 8(1):307-314.

27. AL-Sadawi, A. A.; Al-Ammar M. and Tuwaij, N.S. 2017. Viral Agent That Causing Diarrhoea among Children in Al-Najaf Province, Iraq. World J.Pharma.Res. 6(8):1-11.

28. Al-Dahmoshi, H. O. M.; Shareef, H. K. I.; Al-Khafaji, N. S. K. and Al-Mammori, R. T. O. 2013. Rapid identification of Rotavirus, Adenovirus and Norovirus using Immunochromatography test among infantile diarrhea, Iraq. I.J.S.N. 4(4): 598-602.

29. Alrifai, S.B.; Alsaadi, A.; Mahmood, Y.A.; Ali, A.A. and Al-Kaisi, L.A. 2009. Prevalence and etiology of nosocomial diarrhoea in children <5 years in Tikrit teaching hospital. East. Medit. Heal. J. 15(5):1111-1118.

30. Mohamed, N.S.; Hameed M.F. and Al-Rubai, H.K. 2016. Detection of Norovirus and Rotavirus in Children under Five Years during winter 2012-2013 in Mosul Province. J. Al-Nahrain Univ. 19(3):101-107.

31. Alani, Q.A.; Ai-Rawi, S.A. Salih, A.K. and Al-Mawla, S.O.G. 2012. Common Rota Virus Gastroenteritis in Children under 5 Years in Maternity and Children Teaching Hospital, western Iraq. Anb. Med. J. 10(1):1-7

32. Mero, W.M.S.; Jameel, A.Y. and Amidy, K.S.Kh. 2015. Microorganisms and viruses causing diarrhea in infants and primary school children and their relation with age and sex in Zakho city, Kurdistan Region, Iraq. Int. J. Res. Med. Sci. 3(11):3266-3273.

33. Ahmed, H.M.; Coulter, J.B.S.; Nakagomi, Hart, O.C.A.; Zaki, J.M.; Al-Rabaty, A.A.; Dove, W. and Cunliffe, N.A. 2006. Molecular Characterization of Rotavirus Gastroenteritis Strains, Iraqi Kurdistan. Emerg.Infec.Dis. 12(5):824-826.

34. Hussan, B.M. (2012). Determination of the prevalence of viral etiology of diarrhea in children less than 5 years of age in Baghdad province. Q.M.J. 8(14):163-176.

35. Mahmood, D.B.; Al-suhail, R.; Al-Hamdani, F.G. and Salih, R.A. 2015. Detection of Adenovirus antigen and its correlation with clinical signs among children with gastroenteritis. Iraqi J. Sci. 56(2):1633-1638.

36. Johargy, A.; Ghazi, H. and Mumenah, A. 2010. Frequency of viral, bacterial and parasitic enteropathogens among young children with acute diarrhoea in Saudi Arabia. J. Pak. Med. Assoc. 60(6):456-459.

37. Amara, M.S.C.; Estevam, G.K.; Penatti, M.; Lafontaine, R.; Lima, I.C.G.; Spada, P.K.P.; Gabbay, Y.B. and Matos, N.B. 2015. The prevalence of norovirus, astrovirus and Adenovirus infections among hospitalized children with acute gastroenteritis in Porto Velho, state of Rondônia, western Brazilian Amazon. Mem Inst Oswaldo Cruz, Rio de Janeiro: 1-7.

38. Salman, Y.J. and Salih, L.A. 2013. Detection of Some Microbial Infectious Agents among Children Aging Below Two Years in Kirkuk City. J.Kirkuk med.coll. 1(1): 52-60.

39. Liu, L.; Qian, Y.; Zhang, Y.; Zhao, L.; Jia, L. and Dong, H. 2016 Epidemiological aspects of Rotavirus and Adenovirus in hospitalized children with diarrhea: a 5-year survey in Beijing. B.M.C. Infect Dis. 16:508:1-7.

40. Tran, A.; Talmud, D.; Lejeune, B.; Jovenin, N.; Renois, F.; Payan, C.; Leveque, N. and Andreoletti L., 2010. Prevalence of Rotavirus, Adenovirus, Norovirus, and Astrovirus Infections and Coinfections among Hospitalized Children in Northern France. J.Clin.Microbiol. 48(5):1943-1946.

41. Oyinloye, S.O.; Idika, J.; Abdullahi, M.; Lawan, M.A.; Dahiru, A. and Salihu, A. 2017. Prevalence of Rotavirus Infection in Infants and Young Children with Gastroenteritis in Two North-East States, Nigeria. British J. Med. Med. Res. 20(2):1-7.

42. Gültepe, B.; Güdücüoğlu, H.; Çıkman, A.; Parlak, M. and Berktaş, M. 2013. Prevalence of Rotavirus and Adenovirus Gastroenteritis Observed Around the Van. Sakarya M.J. 3(3):131- 134.

43. Sharifi-Rad, J.; Alfatemi, S.M.H.; Sharifi-Rad, M. and Miri A. 2015. Frequency of Adenoviruses, Rotaviruses and Noroviruses among Diarrhea Samples Collected From Infants of Zabol, Southeastern Iran. Jundishapur J. Microbiol. 8(3): e15440.

44. Khalil, M.; Azhar, E.; Kao, M.; Al-Kaiedi, N.; Alhani, H.; Al-Olayan, I.; Pawinski, R.; Gopala, K.; Kandeil, W.; Anis, S.; Van Doorn, L. and DeAntonio, R. 2015. Gastroenteritis attributable to Rotavirus in hospitalized Saudi Arabian children in the period 2007–2008. Clin. Epidemiol. 7:129-137.

45. Mayindou, G.; Ngokana, B.; Sidibe, A.; Mounde, V.; Koukouikila-Koussounda, F.; Vouvoungui, J.C.; Nolna, S.K.; Velavan, T.P. and Ntoumi, F. 2016. Molecular Epidemiology and Surveillance of Circulating Rotavirus and Adenovirus in Congolese Children with Gastroenteritis. J. Med. Vir. 88(4):596-605.

46. Abdulrazzaq, A.; Aljeboory, S.K.; Abdulkareem, S. and Klena, J. 2011. Two different diagnostic methods for detection of rotavirus in Iraqi young Children. Al-Anbar. J. Vet. Sci. 4(1):50-55.

47. Saderi, H.; Roustai, M.H.; Sabahi, F.; Sadeghizadeh, M.; Owlia, P. and De-Jong, J.C. 2002. Incidence of enteric Adenovirus gastroenteritis in Iranian children. J. Clin. Virol. 24(1-2):1-5.

48. Kim, J.; Lee, S.; Ko, D.; Hyun, J.; Kim, H.; Song, W. and Kim, H.S. 2017. Associations of Adenovirus Genotypes in Korean Acute Gastroenteritis Patients with Respiratory Symptoms and Intussusception. Bio. Med. Research International. Article ID 1602054, 1-6.

49. Nitiema, L.W.; Nordgren, J.; Ouermi, D.; Dianou, D.; Traore, A.S.; Svensson, L. and Simpore, J. 2011. Burden of Rotavirus and other enteropathogens among children with diarrhea in Burkina Faso. Inter. J. Infec. Dis. 15(9):646-652.