Document Type

Research Article


Pseudomonas aeruginosa has become a major cause of life-threatening nosocomial infections that can rapidly develop resistance to multiple antimicrobial agents, mostly due to its ability to produce extended-spectrum β-lactamase (ESBL),AmpC and metallo-β-lactamase(MBL).Biofilm formation is another important factor contributes to the chronicity of infections as they reduce susceptibility to antimicrobial agents. This study was aimed to find prevalence of ESBL and AmpC β-lactamases production and biofilm formation in burn isolates of P. aeruginosa. Further we find out the potential to form biofilm in relation to antibiotic resistance and β-lactamase productions.A total of 90 isolates of P. aeruginosa were isolated from burn samples .We applied Vitek-2 automated system at Rozhawa hospital as a panel of antimicrobial agents. Microtiter plate assay was chosen to detect the biofilm formation. Combined disk diffusion method and AmpC disc test were followed for phenotypic detection of ESBL and AmpC production, respectively. Of the 90 P. aeruginosa burn isolates, 31.1% of isolates formed biofilms ,the majority of which (71.4%) were moderate biofilms. ESBL production 58 (64.4%) was found to be the predominant resistance mechanism followed by AmpC β-lactamase production 46(51.1%). Production of biofilm were higher in the ESBL,AmpC and ESBL+AmpC β-lactamases producers (34.4%,32.6%and37.9%) respectively,compared with the ESBL,AmpC and ESBL+AmpC β-lactamases non-producers (25%,31.8%and 27.8%) respectively.A significant association was found between the degree of biofilm formation and betalactamase production in P. aeruginosa.(P<0.05) The biofilm producing P.aeruginosa were significantly (P<0.01) more resistant compared to biofilm non producers. Biofilm production was strongly correlated to antibiotic resistance .

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